Abstract Paper
| Title | : Synthesis of colloidal copper nanoparticles and their ability to modify the DNA structure in a programmable pathway |
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| Author(s) | : Natarajan Prabakaran, Sepperumal Murugesan, Periakaruppan Athappan |
| Article Information | : Volume 2 - Issue 2 (December- 2018) , 40-47 |
| Affiliation(s) | : School of Chemistry, Madurai Kamaraj University, Madurai-21 |
| : Assistant Professor, Department of Chemistry, Yadava college, Madurai-14: 9787147490: 0452-2697093. |
Abstract :
Synthesis of tetrapodal structured cationic cetyltrimethylammonium bromide stabilized copper nanoparticles (CTAB-CuNPs) and spherical shaped anionic sodium dodecyl sulphate stabilized copper nanoparticles (SDS-CuNPs), which are stable for more than 100 days in aqueous solutions, by chemical reduction under sonication is reported. The differences in their binding with DNA and the changes in the structural morphology on a programmable way were studied through UV-vis spectroscopic, atomic force microscopic (AFM), scanning electron microscopic (SEM), transmission electron microscopic (TEM), emission and electrophoresis techniques. The circular dichroic (CD) spectral studies reveal the significant effect of CTAB-CuNPs in transforming B to Z conformation in CT-DNA. At lower concentrations, copper nanoparticles form spaghetti/ellipsoid like structures with DNA, while at higher concentrations cleaves the DNA and form discrete chain-like assemblies. The emission and gel electrophoresis studies also support these findings.
| Keywords | : Copper nanoparticles; nanoparticle-DNA interaction; tetrapodal assembly; groove binding; CD spectroscopy |
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| Document Type | : Research Paper |
| Publication date | : December 10, 2018 |